If the Base Sequence of Template Strand Reads Gccattac, What Is the Base Sequence of the Mrna?

Essential Biology Notes:

one. Distinguish between sense and antisense strands in Dna.

The antisense strand is complementary to the sense strand. The antisense strand is besides known as the template strand, as it is used for transcription , to brand mRNA

mRNA corresponds with the antisense strand, with the exception of the base T , which is replaced with U . mRNA is complementary to the sense strand.

2. Draw the role of the antisense strand in transcription.

Antisense strand is complementary to the sense strand. It is the template strand to produce mRNA with the same genetic codes as the sense strand.

3. State the direction of transcription and draw a simple diagram to show the addition of an RNA molecule to a growing mRNA strand.

Transcription is in v' - 3' direction.

iv. Outline the roles of the following regions of Deoxyribonucleic acid in translation:

Promoter region

Site where RNA polymerase attaches and unwinds DNA

Coding region

Transcribed section

Terminator

End bespeak in transcription

five. Explain the process of translation in prokaryotes, including the post-obit: promoter region, RNA polymerase, five'-three' direction, free nucleoside triphosphates, complementary base pairing, terminator region.

                   (Transcription vocabulary definitions)

  (Stride ane)

   (Step 2)

    (Step iii)


6. Distinguish betwixt introns and exons in eukaryotic Dna (DNA structure revision).

Introns - non coding regions that need to be removed before translation

    Exons - mature mRNA ready for translation

vii. State one method of post-transcription modification of mRNA in eukaryotes.

introns are removed to form mature mRNA, using spliceosomes

8. Label the following regions on this tRNA molecule, stating the role of each:

a.

 acceptor loop

 site attaches to amino acrid at 3' last end

b.

hydrogen bonds

 attaches base-pairing

c.

 anticodon

 attaches to mRNA codons

nine. tRNA needs to be activated in order to pick up the corresponding amino acid.

Explain how tRNA is activated, including the role of a specific tRNA activating enzyme, ATP and amino acids.

  • tRNA delivers amino acrid to polypeptide chain
  • tRNA looses amino acid
  • tRNA-activating enzyme will pick upward new amino acid - uses ATP
  • tRNA and amino acid are specific - only pick up corresponding shape and chemical properties

10. Using a diagram, outline the structure of a ribosome, including protein and RNA composition, large and pocket-size subunits, 3 tRNA bounden sites and mRNA binding sites.

11. Depict polysomes and land their part.

Polysomes - group of ribosomes moving along the same mRNA

    Office - elongate polypeptide

12. Land the management of motion of the ribosome forth the mRNA strand (the direction of translation).

The direction of movement of the ribosomes forth the mRNA strand is in the v' to three' direction.

13. Explicate the procedure of translation in 4 stages:

1. Initiation

Keywords: mRNA, ribosome, small-scale subunit, large subunit, start codon, AUG, tRNA

Explanation:


2. Elongation

Keywords: amino acids, peptide bail, transfer, anticodon, tRNA

Explanation:


3. Translocation

Keywords: tRNA, mRNA, codon, release

Explanation:


iv. Termination

Keywords: stop codon, release/ detachment

Explanation:


14. Show how a dipeptide is formed through condensation, labeling the peptide bond.

fifteen. Distinguish between polypeptides produced by gratis ribsomes and those produced by bound ribosomes, such as in the RER. (Revision: exocytosis)

· Free ribosomes: used inside the cell

· RER-bound ribosomes: for consign out of the cell or lysosomes

Essential Biology (embedded without related content):


seylerdritte.blogspot.com

Source: https://www.sites.google.com/site/bisbiology2012/home/whatup-hl/7-3-7-4-transcription-translation

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